MIIP Polyclonal Antibody

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti MIIP Polyclonal Antibody, Unconjugated (bs-5842R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$L1 rat kidney lysates L2 rat brain lysates probed with Anti MIIP Polyclonal Antibody, Unconjugated (bs-5842R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 43kD. Observed band size: 35kD, 43kD$

L1 rat kidney lysates L2 rat brain lysates probed with Anti MIIP Polyclonal Antibody, Unconjugated (bs-5842R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 43kD. Observed band size: 35kD, 43kD

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Horse
Rabbit

Overview
Catalog #	bs-5842R
Product Name	MIIP Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MIIP
Immunogen Range	251-350/388
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	60672
Swiss Prot	Q5JXC2
Subcellular location	Cytoplasm, Nucleus
Synonyms	IIP45; Migration and invasion-inhibitory protein; IGFBP2-binding protein; Invasion-inhibitory protein 45; MIIP
Background	Inhibits glioma cells invasion and down-regulates adhesion- and motility-associated genes such as NFKB2 and ICAM1. Exhibits opposing effects to IGFBP2 on cell invasion.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200