ANGPTL3 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human kidney labeled with Anti ANGPTL3\/ANG5 Polyclonal Antibody, Unconjugated (bs-5918R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human kidney labeled with Anti ANGPTL3\/ANG5 Polyclonal Antibody, Unconjugated (bs-5918R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

HepG2 lysates probed with ANGPTL3 Polyclonal Antibody, Unconjugated (bs-5918R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Human HL-60 cell Lysates; Lane 2: Human HepG2 cell Lysates; Lane 3: Human 293T cell Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human MOLT4 cell Lysates. Probed with ANGPTL3 polyclonal Antibody, unconjugated (bs-5918R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HL-60 cell Lysates; Lane 2: Human HepG2 cell Lysates; Lane 3: Human 293T cell Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human MOLT4 cell Lysates. Probed with ANGPTL3 polyclonal Antibody, unconjugated (bs-5918R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human

Overview
Catalog #	bs-5918R
Product Name	ANGPTL3 Polyclonal Antibody
Applications	WB ELISA
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ANG5/ANGPTL3
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	27329
Swiss Prot	Q9Y5C1
Subcellular location	Secreted
Synonyms	ANL3; ANG-5; FHBL2; ANGPT5; Angiopoietin-related protein 3; Angiopoietin-5; Angiopoietin-like protein 3; ANGPTL3; UNQ153/PRO179
Background	Angiopoietin-like protein 3 (Angptl3) functions as a potent lipoprotein lipase inhibitor and is an important component of plasma triglyceride homeostasis. Angptl3 also plays a role in adipose formation and angiogenesis through its interaction with integrin ?v)beta(3). It is secreted by the liver and is functionally defined by the C-terminal fibrinogen (FBN)-like domain and an N-terminal coiled-coil domain. Angptl3 regulates circulating triglyceride levels during different nutritional states thereby mediating the feeding/fasting cycle. A deficiency of Angptl3 results in abnormally low lipid levels, and a repression of the protein may be protective against atherosclerosis. Angptl3 may also play an important role in hyperlipidemia in diabetes.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000