ABI3 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-ABI3 Polyclonal Antibody, Unconjugated (bs-5985R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Tissue\/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37\u2103 for 20 min; Incubation: Anti-ABI3 Polyclonal Antibody, Unconjugated(bs-5985R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining$

Tissue\/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37\u2103 for 20 min; Incubation: Anti-ABI3 Polyclonal Antibody, Unconjugated(bs-5985R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

$25 ug total protein per lane of various lysates (see on figure) probed with ABI3 polyclonal antibody, unconjugated (bs-5985R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ABI3 polyclonal antibody, unconjugated (bs-5985R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Rabbit

Overview
Catalog #	bs-5985R
Product Name	ABI3 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ABI3
Immunogen Range	151-250/366
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51225
Swiss Prot	Q9P2A4
Subcellular location	Cytoplasm
Synonyms	NESH; SSH3BP3; ABI gene family member 3; New molecule including SH3; ABI3
Background	May inhibit tumor metastasis (By similarity). In vitro, reduces cell motility.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500