Histone H3 (tri methyl K4, serotonyl Q5) Polyclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Histone H3 (tri methyl K4, serotonyl Q5) polyclonal antibody, unconjugated (bs-25934R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Histone H3 (tri methyl K4, serotonyl Q5) polyclonal antibody, unconjugated (bs-25934R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature\uff0cfollowed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Histone H3 (tri methyl K4, serotonyl Q5) antibody (bs-60081R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature\uff0cfollowed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Histone H3 (tri methyl K4, serotonyl Q5) antibody (bs-60081R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-60081r
Product Name	Histone H3 (tri methyl K4, serotonyl Q5) Polyclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P68431
Subcellular location	Nucleus
Synonyms	H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; H3/b; H3C1; H3FB; HIST1H3D; H3.1; H3/c; H3FC; HIST1H3C; H3/d; H3FD; HIST1H3E; H3.f; H3/f; H3FF; HIST1H3I; H3/h; H3FH; HIST1H3G; H3/j; H3FJ; HIST1H3J; H3/k; H3F1K; H3FK; HIST1H3H; H3/l; H3FL; HIST1H3B; H3/i; H3FI; HIST1H3F; H3.2-221; H3c13; H3c14; H3c15; Hist1; H3-143; H3-b; H3-53; H3-f; H3-291; H3-H; H3.1-221; H3 histone; Hist1h2ail; Hist1h2ail1; Hist2h3c; Hist3h3; H31_HUMAN; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3FC HIST1H3C; H33_HUMAN; H3-3A; H3-3B; H3.3A; H3F3; H3F3A; H3.3B; H3F3B; H31T_HUMAN; H3-4; H3/t; H3t; H3/g; Histone H3.4; H3FT; H3C_HUMAN; H3-5; Histone H3.5; H3F3C; H32_HUMAN; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; Histone H3/m; Histone H3/o; HIST2H3A; H3F2; H3FM; HIST2H3D; H31_MOUSE; H3a; H3g; H3.1-291; H3h; H3.1-I; H3i; H32_MOUSE; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3.2; H3b; H3f; H3.2-616; Hist2h3b; H3.2-615; Hist2h3c1; Hist2h3ca1; H3.2-614; Hist2h3c2; Hist2h3ca2; H31_RAT; H3 clustered histone 1; H3 histone family, member A; histone 1, H3a; histone cluster 1, H3a; histone cluster 1 H3 family member a; H3C1 \| Histone H3 (tri-methyl-K4/serotonyl-Q5); H3K4me3Q5ser; me3/ser-Histone H3; tri-methyl/serotonyl-Histone H3; me3/ser-H3; H3C1 \| Histone H3 (tri-methyl-Lys4/serotonyl-Q5)
Application Dilution
WB	1:300-5000