HPGD Polyclonal Antibody

Lovo cell lysates probed with Prostaglandin dehydrogenase 1 Polyclonal Antibody, Unconjugated (bs-6051R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1:SW480 cell lysates probed with Prostaglandin dehydrogenase 1 Polyclonal Antibody, Unconjugated (bs-6051R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Prostaglandin dehydrogenase 1 Polyclonal Antibody, Unconjugated (bs-6051R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Prostaglandin dehydrogenase 1 Polyclonal Antibody, Unconjugated (bs-6051R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-6051r-TR
Product Name	HPGD Polyclonal Antibody
Applications	WB IHC-P IHC-F IF FLOW-CYT
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Prostaglandin dehydrogenase 1
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3248
Swiss Prot	P15428
Subcellular location	Cytoplasm
Synonyms	PGDH_HUMAN; 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; EC:1.1.1.141; 15-PGDH; 15 PGDH; 15PGDH; Eicosanoid/docosanoid dehydrogenase [NAD(+)](EC:1.1.1.-; EC:1.1.1.232; Prostaglandin dehydrogenase 1; Short chain dehydrogenase/reductase family 36C member 1; PGDH1; SDR36C1; PGDH; PGDH1; PHOAR1;
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500
Flow-Cyt	Flow-Cyt=1:10-50