GNAQ Polyclonal Antibody

$Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-GNAQ\/G\u03b1 q Polyclonal Antibody, Unconjugated (bs-6152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-GNAQ\/G\u03b1 q Polyclonal Antibody, Unconjugated (bs-6152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-GNAQ\/G\u03b1 q Polyclonal Antibody, Unconjugated (bs-6152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GNAQ\/G\u03b1 q Polyclonal Antibody, Unconjugated (bs-6152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti GNAQ\/G\u03b1 q Polyclonal Antibody, Unconjugated (bs-6152R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.$

Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti GNAQ\/G\u03b1 q Polyclonal Antibody, Unconjugated (bs-6152R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.

Mouse ovary lysates probed with GNAQ Polyclonal Antibody, Unconjugated (bs-6152R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

SY5Y cell lysates probed with GNAQ Polyclonal Antibody, Unconjugated (bs-6152R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-6152R
Product Name	GNAQ Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GNAQ
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2776
Swiss Prot	P50148
Subcellular location	Nucleus, Cell membrane
Synonyms	GAQ; SWS; CMC1; G-ALPHA-q; Guanine nucleotide-binding protein G(q) subunit alpha; Guanine nucleotide-binding protein alpha-q; GNAQ
Background	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200