Prostatic Acid Phosphatase Antibody

$Paraformaldehyde-fixed, paraffin embedded human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Prostatic Acid Phosphatase Antibody (bs-6293R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Prostatic Acid Phosphatase Antibody (bs-6293R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Horse

Overview
Catalog #	bs-6293R
Product Name	Prostatic Acid Phosphatase Antibody
Applications	WB IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Prostatic Acid Phosphatase
Immunogen Range	21-120/386
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	52
Swiss Prot	P24666
Subcellular location	Secreted, Cell membrane
Synonyms	HAAP; Low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; LMW-PTPase; Adipocyte acid phosphatase; Low molecular weight cytosolic acid phosphatase; Red cell acid phosphatase 1; ACP1
Background	Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200