CHCHD2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CHCHD2 Polyclonal Antibody, Unconjugated (bs-6407R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CHCHD2 Polyclonal Antibody, Unconjugated (bs-6407R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
IHC-P
IHC-F

Reactivity

Mouse
GuineaPig

Predicted Reactivity

Human
Rat
Dog
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-6407R
Product Name	CHCHD2 Polyclonal Antibody
Applications	WB IHC-P IHC-F
Reactivity	Mouse, GuineaPig
Predicted Reactivity	Human, Rat, Dog, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CHCHD2 (96-130aa)
Immunogen Range	96-130/151
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51142
Subcellular location	Cytoplasm
Synonyms	mitochondrial; 16.7kD protein; Aging Associated Gene 10 Protein; Aging-associated gene 10 protein; C7orf17; CHCH2_HUMAN; CHCHD 2; CHCHD2; Coiled Coil Helix Coiled Coil Helix Domain Containing 2; Coiled coil helix coiled coil helix domain containing protein 2, mitochondrial; Coiled-coil-helix-coiled-coil-helix domain-containing protein 2; HCV NS2 trans regulated protein; HCV NS2 trans-regulated protein; NS2TP.
Background	CHCHD2
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500