KCNN4 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 50kD. Observed band size: 45kD, 50kD.

$Lane 1: Human U-2 OS cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human LOVO cell lysates; Lane 6: Human HepG2 cell lysates probed with KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Human U-2 OS cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human LOVO cell lysates; Lane 6: Human HepG2 cell lysates probed with KCNN4 Polyclonal Antibody, Unconjugated (bs-6675R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

WB
ELISA

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-6675r-TR
Product Name	KCNN4 Polyclonal Antibody
Applications	WB ELISA
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KCNN4
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3783
Swiss Prot	O15554
Subcellular location	Cell membrane
Synonyms	hIKCa1; IK1; IKCa1; Intermediate conductance calcium activated potassium channel protein 4; Intermediate conductance calcium-activated potassium channel protein 4; KCa3.1; KCa4; KCNN 4; Kcnn4; KCNN4_HUMAN; Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4; Putative Gardos channel; SK4; SKCa 4; SKCa4.
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000