DsbA-L (Disulfide-bond-A oxidoreductase-like protein) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70073r |
| Product Name | DsbA-L (Disulfide-bond-A oxidoreductase-like protein) Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~25 kDa DsbA-L protein. It is suggested that you add 1% BSA to the antibody dilution buffer. |
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Full length recombinant mouse DsbA-L protein. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | whole antiserum |
| Storage Buffer | whole antiserum in 40% glycerol |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Swiss Prot | Q9DCM2 |
| Synonyms | DSBA like thioredoxin domain containing protein antibody, Glutathione S-transferase kappa 1, EC:2.5.1.18, GST 13-13, GST class-kappa, GST kappa, GSTK1-1, mGSTK1, Glutathione S-transferase subunit 13 |
| Background | Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution (Gao F et al., 2009). Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis (Liu et al., 2008). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |