FMRP (Ser499) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70090r |
| Product Name | FMRP (Ser499) Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling is completely eliminated by treatment with _-phosphatase. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Predicted Reactivity | Dog, Bovine, Sheep, Zebrafish, Xenopus, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 of rat FMRP, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser499 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target | |
| Gene ID | 24948 |
| Swiss Prot | Q80WE1 |
| Synonyms | FMR 1 antibody, Fmr1 antibody, Fmr1 gene antibody, FMR1_HUMAN antibody, FMRP antibody, Fragile X mental retardation 1 antibody, Fragile X mental retardation 1 protein antibody, Fragile X mental retardation protein 1 antibody, Fragile X mental retardation protein antibody, fragile X mental retardation syndrome-related protein 1 antibody, fragile X mental retardation autosomal homolog 1 antibody, FRAXA antibody, fxr1 antibody, MGC87458 antibody, POF antibody, POF1 antibody, Protein FMR-1 antibody, Protein FMR1 antibody, wu:fb16f11 antibody, wu:fd18c10 antibody, zgc:66226 antibody |
| Background | Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser-499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |