MEK1/2 (Ser218,222) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70152r |
| Product Name | MEK1/2 (Ser218,222) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218/222. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Bovine, Chicken, Zebrafish, Xenopus, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 of human MEK1/2, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser218,222 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 5604 |
| Swiss Prot | Q02750 |
| Synonyms | AA589381 antibody, CFC3 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 2 antibody, EC 2.7.12.2 antibody, ERK activator kinase 1 antibody, ERK activator kinase 2 antibody, FLJ26075 antibody, MAP kinase kinase 1 antibody, MAP kinase kinase 2 antibody, MAP2K1 antibody, MAP2K2 antibody, MAPK/ERK kinase 1 antibody, MAPK/ERK kinase 2 antibody, MAPKK 1 antibody, MAPKK1 antibody, MAPKK2 antibody, MEK 1 antibody, MEK1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, Mitogen activated protein kinase kinase 2 antibody, Mitogen-activated protein kinase kinase 2, p45 antibody, MK2 antibody, MKK 1 antibody, MKK 2 antibody, MKK1 antibody, MKK2 antibody, MP2K1_HUMAN antibody, PRKMK 1 antibody, PRKMK 2 antibody, Prkmk1 antibody, Prkmk2 antibody, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody, Protein kinase, mitogen-activated, kinase 1 antibody, Protein kinase, mitogen-activated, kinase 2 antibody |
| Background | MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). |
| Application Dilution | |
| WB | 1:300-5000 |