Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70178r |
| Product Name | Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~52 kDa nAChR_4 protein. |
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Fusion protein from the cytoplasmic loop of the _4 subunit of mouse nAChR. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target | |
| Gene ID | 108015 |
| Swiss Prot | Q8R493 |
| Synonyms | acetylcholine receptor nicotinic beta 4 (neuronal) antibody, ACHB4_HUMAN antibody, AChR antibody, Cholinergic receptor nicotinic beta 4 antibody, Cholinergic receptor nicotinic beta polypeptide 4 antibody, cholinergic receptor nicotinic beta polypeptide 4 antibody, Chrnb4 antibody, Neuronal acetylcholine receptor subunit beta-4 antibody, Neuronal nicotinic receptor beta 4 subunit antibody |
| Background | Nicotinic acetylcholine receptors (nAChRs) are ionotropic, cholinergic receptors that are divided into 2 types; muscle type and neuronal type. Neuronal nAChRs are pentameric ion channels consisting of 5 identical (homopentamers) or different (heteropentamers) subunits. Heteropentameric neuronal nAChRs mediate fast synaptic transmission in the autonomic nervous system. The predominant hetero-oligomeric nAChR in the CNS contain the subunits _4_2, whereas _3_4 prevail in the PNS. However, the expression of these subunits varies not only by region but also during development (Scholze et al 2011). In the brain, _2-containing receptors greatly outnumber receptors that contain _4 (McGehee & Role, 1995; Albuquerque, et al., 2009), and in most brain regions, targeted deletion of the _2 subunit virtually abolishes [3H]-epibatidine binding and receptor autoradiography (Zoli, et al., 1998) due to the absence of a _ subunit required to form functional nAChRs (Champtiaux & Changeux, 2004). |
| Application Dilution | |
| WB | 1:300-5000 |