Troponin I (cardiac) Ser150 Antibody

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Non-Human Primate

Overview
Catalog #	bs-70295r
Product Name	Troponin I (cardiac) Ser150 Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with _-phosphatase treatment.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser150
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol.
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Gene ID	21954
Swiss Prot	P48787
Synonyms	cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody
Background	Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser-23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser-23 and Ser-150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser-150 over Ser-23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).
Application Dilution
WB	1:300-5000