_-Catenin (Tyr-550), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70387r |
| Product Name | _-Catenin (Tyr-550), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | This antibody was cross-adsorbed to both phospho-tyrosine coupled to agarose and to dephosphorylated _-Catenin (Tyr-550) peptide before affinity purification using phospho-_-Catenin (Tyr-550) peptide (without carrier). |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phospho-_-Catenin (Tyr-550) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 550 of human _-Catenin. |
| Modification Site | Tyr-550 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target | |
| Swiss Prot | P14923 |
| Synonyms | Plakoglobin, JUP, Desmoplakin III, Desmoplakin-3, catenin gamma1 |
| Background | Plakoglobin (_-Catenin) is a catenin family member identified as a component of desmosomes. _-Catenin has high homology to _-catenin and, like _-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of _-catenin and at least one molecule of _-catenin and _-Catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of _-Catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for _-Catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues in _-Catenin can modify its interactions with other proteins. Phosphorylation of tyrosine 644 decreases _-Catenin association with _-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate tyrosine 550, which increases _-Catenin binding to _-catenin. Thus, tyrosine phosphorylation may be important for regulation of _-Catenin protein-protein interactions within desmosomal complexes. |
| Application Dilution | |
| WB | 1:300-5000 |