CrkL (C-terminus) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70434r |
| Product Name | CrkL (C-terminus) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 38 kDa* protein corresponding to the molecular mass of CrkL on SDS-PAGE immunoblots of human K562 cells, mouse brain tissue, and NGF-differentiated rat PC12 cells. This peptide sequence is well conserved in mouse and rat CrkL, and has less than 50% homology to human Crk II. |
| Reactivity | Human, Mouse, Rat, Chicken, Xenopus |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | CrkL synthetic peptide (coupled to KLH) containing amino acid in the C-terminus of human CrkL. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target | |
| Swiss Prot | P46109 |
| Synonyms | v-crk sarcoma virus CT10 oncogene homolog, CRKII, CRKL |
| Background | The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl. |
| Application Dilution | |
| WB | 1:300-5000 |