SMAD7 (N-terminal region) Antibody

Applications

Reactivity

Human
Mouse
Rat
Xenopus

Overview
Catalog #	bs-70666r
Product Name	SMAD7 (N-terminal region) Antibody
Applications	WB
Specificity	This antibody detects SMAD7 at 46 kDa* on SDS-PAGE immunoblots of human Jurkat cells and mouse C2C12. The antibody can used for western blot and immunohistochemistry.
Reactivity	Human, Mouse, Rat, Xenopus
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	This antibody was raised against amino acids from the N-terminal region of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
Clonality	Polyclonal
Isotype	IgG
Purification	Purified by Protein G.
Storage Buffer	PBS + 0.05% BSA and 0.05% NaN3
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy
Target
Swiss Prot	O15105
Synonyms	MADH7, MADH8, MAD7
Background	SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 3. 5 and 8), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.
Application Dilution
WB	1:300-5000