VG5Q Polyclonal Antibody

$Antigen: bs-7509P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in Microplate Reader by 450nm\\n$

Antigen: bs-7509P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in Microplate Reader by 450nm\\n

$Lane 1: mouse lung lysates Lane 2: mouse intestine lysates probed with Anti VG5Q\/AGGF1 Polyclonal Antibody, Unconjugated (bs-7509R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 79kD. Observed band size: 79kD.$

Lane 1: mouse lung lysates Lane 2: mouse intestine lysates probed with Anti VG5Q\/AGGF1 Polyclonal Antibody, Unconjugated (bs-7509R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 79kD. Observed band size: 79kD.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Predicted Reactivity

Human
Mouse
Rat
Dog
Cow
Pig
Horse
Chicken

Overview
Catalog #	bs-7509R
Product Name	VG5Q Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity	Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human VG5Q/AGGF1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55109
Swiss Prot	Q8N302
Subcellular location	Cytoplasm, Secreted
Synonyms	VG5Q; GPATC7; GPATCH7; HSU84971; HUS84971; Angiogenic factor with G patch and FHA domains 1; Angiogenic factor VG5Q; hVG5Q; G patch domain-containing protein 7; Vasculogenesis gene on 5q protein; AGGF1
Background	Promotes angiogenesis and the proliferation of endothelial cells. Able to bind to endothelial cells and promote cell proliferation, suggesting that it may act in an autocrine fashion.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200