EDG3 Polyclonal Antibody

$Lane 1: Mouse Heart lysates probed with Anti-EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:300 in dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at RT.$

Lane 1: Mouse Heart lysates probed with Anti-EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:300 in dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at RT.

$Lane 1: Rat Cerebrum cell lysates; Lane 2: Mouse Cerebrum cell lysates; Lane 3:Rat Heart cell lysates; Lane 4: Mouse Heart cell lysates probed with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Rat Cerebrum cell lysates; Lane 2: Mouse Cerebrum cell lysates; Lane 3:Rat Heart cell lysates; Lane 4: Mouse Heart cell lysates probed with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-7541r-100l
Product Name	EDG3 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human EDG3
Immunogen Range	145-250/378
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1903
Swiss Prot	Q99500
Subcellular location	Cell membrane
Synonyms	EDG3; LPB3; S1P3; EDG-3; Sphingosine 1-phosphate receptor 3; S1P receptor 3; Endothelial differentiation G-protein coupled receptor 3; Sphingosine 1-phosphate receptor Edg-3; S1P receptor Edg-3; S1PR3
Background	Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Application Dilution
WB	1:5000-10000
ELISA	1:500-2000
IHC-P	1:100-500
IHC-F	1:100-500
IF	1:100-500