CEP55 Polyclonal Antibody

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti CEP55 Polyclonal Antibody, Unconjugated (bs-7742R) followed by conjugation to the secondary antibody and DAB staining

$Mouse testis lysates probed with CEP55 Polyclonal Antibody, Unconjugated (bs-7742R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Mouse testis lysates probed with CEP55 Polyclonal Antibody, Unconjugated (bs-7742R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Sheep
Pig
Horse

Overview
Catalog #	bs-7742R
Product Name	CEP55 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CEP55
Immunogen Range	151-250/464
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55165
Swiss Prot	Q53EZ4
Subcellular location	Cytoplasm
Synonyms	CT111; URCC6; C10orf3; Centrosomal protein of 55 kDa; Cep55; Up-regulated in colon cancer 6
Background	Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200