AKR1A1/ALDR1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: mouse pancreas lysates Lane 2: mouse brain lysates probed with Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-1803R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 36kD. Observed band size: 36kD.$

Lane 1: mouse pancreas lysates Lane 2: mouse brain lysates probed with Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-1803R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 36kD. Observed band size: 36kD.

$Mouse liver lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Mouse liver lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: Mouse Liver cell lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Liver cell lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-7953R
Product Name	AKR1A1/ALDR1 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AKR1A1
Immunogen Range	53-130/325
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10327
Swiss Prot	P14550
Subcellular location	Cytoplasm, Secreted, Cell membrane, Extracellular matrix
Synonyms	Aldo-keto reductase family 1 member A1; Alcohol dehydrogenase [NADP(+)]; Aldehyde reductase; Glucuronate reductase; Glucuronolactone reductase; AKR1A1; ALDR; ALR; AK1A1_HUMAN
Background	Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4-nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D-glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200