COMMD4 Polyclonal Antibody

$paraformaldehyde-fixed, paraffin embedded rat testis tissue; Antigen retrieval with 0.01M citrate buffer, boiling for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-COMMD4 Polyclonal Antibody, Unconjugated (bs-8037R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining$

paraformaldehyde-fixed, paraffin embedded rat testis tissue; Antigen retrieval with 0.01M citrate buffer, boiling for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-COMMD4 Polyclonal Antibody, Unconjugated (bs-8037R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining

Formalin-fixed and paraffin embedded human laryngocarcinoma tissue labeled with Anti-COMMD4 Polyclonal Antibody, Unconjugated(bs-8037R) 1:400 followed by conjugation to the secondary antibody and DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Pig
Horse

Overview
Catalog #	bs-8037R
Product Name	COMMD4 Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Human COMMD4
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	54939
Swiss Prot	Q9H0A8
Subcellular location	Cytoplasm, Nucleus
Synonyms	COMM domain-containing protein 4; COMMD4
Background	May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes (PubMed:21778237). Down-regulates activation of NF-kappa-B.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200