FNDC5 Polyclonal Antibody

$Lane 1: HepG2 cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: K562 cell lysates; Lane 4: Mouse heart lysates probed with FNDC5 Polyclonal Antibody, Unconjugated (bs-8486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: HepG2 cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: K562 cell lysates; Lane 4: Mouse heart lysates probed with FNDC5 Polyclonal Antibody, Unconjugated (bs-8486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Chicken
Rabbit

Overview
Catalog #	bs-8486R
Product Name	FNDC5 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FNDC5 Irisin
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	252995
Swiss Prot	Q8NAU1
Subcellular location	Cytoplasm, Secreted, Cell membrane
Synonyms	FRCP2; irisin; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; FNDC5
Background	Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200