Somatostatin 28 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Somatostatin Polyclonal Antibody, Unconjugated (bs-8877R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Somatostatin Polyclonal Antibody, Unconjugated (bs-8877R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Applications

WB
IHC-P
IF(IHC-P)
IF(IHC-F)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-8877R
Product Name	Somatostatin 28 Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P) IF(IHC-F)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Somatostatin
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6750
Swiss Prot	P61278
Subcellular location	Secreted
Synonyms	SMST; Somatostatin; Growth hormone release-inhibiting factor; SST
Background	Somatostatin inhibits the release of somatotropin.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200