RNF123 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-RNF123 Polyclonal Antibody, Unconjugated (bs-9249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RNF123 Polyclonal Antibody, Unconjugated (bs-9249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse brain probed with Anti RNF123 Polyclonal Antibody, Unconjugated (bs-2530R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.

$HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with RNF123 Polyclonal Antibody(bs-9249R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).$

HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with RNF123 Polyclonal Antibody(bs-9249R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9249R
Product Name	RNF123 Polyclonal Antibody
Applications	WB IHC-P IHC-F IF FLOW-CYT
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RNF123
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	63891
Swiss Prot	Q5XPI4
Subcellular location	Cytoplasm
Synonyms	KPC1; FP1477; E3 ubiquitin-protein ligase RNF123; Kip1 ubiquitination-promoting complex protein 1; RING finger protein 123; RNF123
Background	Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	IFIHC-P1:50-200
IF
Flow-Cyt