Kininogen-1 light chain Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kininogen-1 light chain Polyclonal Antibody, Unconjugated (bs-9504R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kininogen-1 light chain Polyclonal Antibody, Unconjugated (bs-9504R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9504R
Product Name	Kininogen-1 light chain Antibody
Applications	ELISA IHC-P IHC-F IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HMW Kininogen-1 light chain
Immunogen Range	551-644/644
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3827
Synonyms	Alpha 2 thiol proteinase inhibitor; HMW Kininogen light Chain; Alpha-2-thiol proteinase inhibitor; Fitzgerald factor; High molecular weight kininogen; HMWK; Ile-Ser-Bradykinin; Kallidin I; Kallidin II; Kininogen 1; KNG1; KNG1_HUMAN; Low molecular weight growth-promoting factor; Williams-Fitzgerald-Flaujeac factor.
Background	This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009].
Application Dilution
ELISA	WB1:300-5000
IHC-P	1:200-400
IHC-F	IFIHC-P1:50-200
IF