CTH Polyclonal Antibody

$Lane 1: Mouse liver lysates probed with CTH Polyclonal Antibody, Unconjugated (bs-9515R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse liver lysates probed with CTH Polyclonal Antibody, Unconjugated (bs-9515R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37\u02daC.

$Lane 1: K562 lysates probed with CTH Polyclonal Antibody, Unconjugated (bs-9515R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37\u02daC.$

Lane 1: K562 lysates probed with CTH Polyclonal Antibody, Unconjugated (bs-9515R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Rabbit

Overview
Catalog #	bs-9515R
Product Name	CTH Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cystathionine gamma lyase
Immunogen Range	51-150/405
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1491
Synonyms	CGL_HUMAN; CTH; Cystathionine gamma lyase; Cystathionine gamma-lyase; Cysteine desulfhydrase; Gamma cystathionase; Gamma-cystathionase; Homoserine deaminase; Homoserine dehydratase; MGC9471; CSE; Cystathionase.
Background	Catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure.Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200