CXorf21 Polyclonal Antibody

$HL-60 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXorf21 Polyclonal Antibody, ALEXA FLUOR\u00ae 647 Conjugated (bs-9552R-AF647) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).$

HL-60 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXorf21 Polyclonal Antibody, ALEXA FLUOR\u00ae 647 Conjugated (bs-9552R-AF647) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Applications

FCM
IHC-P
IF(IHC-P)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Rabbit

Overview
Catalog #	bs-9552r
Product Name	CXorf21 Polyclonal Antibody
Applications	FCM IHC-P IF(IHC-P)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CXorf21
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	80231
Swiss Prot	Q9HAI6
Synonyms	Chromosome X open reading frame 21; FLJ11577; Hypothetical protein LOC80231; Uncharacterized protein CXorf21; CX021_HUMAN.
Background	The X and Y chromosomes are the human sex chromosomes. Chromosome X consists of about 153 million base pairs and nearly 1,000 genes. The combination of an X and Y chromosome lead to normal male development while two copies of X lead to normal female development. There are a number of conditions related to an unsual number and combination of sex chromosomes being inherited. More than one copy of the X chromosome with a Y chromosome causes Klinefelter's syndrome. A single copy of X alone leads to Turner's syndrome. More than 2 copies of the X chromosome, in the absence of a Y chromosome, is known as Triple X syndrome. Color blindness, hemophilia, and Duchenne muscular dystrophy are well known X chromosome-linked conditions which affect males more frequently as males carry a single X chromosome. The CXorf21 gene product has been provisionally designated CXorf21 pending further characterization.
Application Dilution
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200