RBM20 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat heart labeled with Anti-RBM20 Polyclonal Antibody, Unconjugated (bs-9606R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: K562 cell lysates; Lane 2: A549 cell lysates probed with RBM20 Polyclonal Antibody, Unconjugated (bs-9606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RBM20 Polyclonal Antibody(bs-9606R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RBM20 Polyclonal Antibody(bs-9606R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9606R
Product Name	RBM20 Polyclonal Antibody
Applications	WB IHC-P IHC-F IF FLOW-CYT
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RBM20
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	282996
Swiss Prot	Q5T481
Subcellular location	Nucleus
Synonyms	RNA-binding protein 20; RNA-binding motif protein 20; RBM20
Background	RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	IFIHC-P1:50-200
IF
Flow-Cyt