FAM29A Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-FAM29A Polyclonal Antibody, Unconjugated (Cbs-9723R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining$

Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-FAM29A Polyclonal Antibody, Unconjugated (Cbs-9723R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Rabbit

Overview
Catalog #	bs-9723R
Product Name	FAM29A Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FAM29A/HAUS6
Immunogen Range	301-400/955
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	Dgt6; Dim gamma tubulin homolog; Family with sequence similarity 29 member A; FLJ20060; HAUS augmin like complex subunit 6; HAUS6; KIAA15741; HAUS6_HUMAN.
Background	The human augmin complex (HAUS) is an evolutionarily conserved 8-subunit protein complex that was initially discovered in Drosophila. The HAUS complex is essential for microtubule generation, centrosome integrity, mitotic spindle assembly and completion of cytokinesis. HAUS6 (HAUS augmin-like complex, subunit 6), also known as FAM29A or Dgt6, is a 955 amino acid component of the augmin complex. Required for mitotic progression, HAUS6 localizes to cytoplasm, cytoskeleton, mitotic spindle microtubules and interphase centrosomes, and undergoes post-translational phosphorylation following mitosis on multiple serine and threonine residues. HAUS6 exists as two alternatively spliced isoforms that are encoded by a gene located on human chromosome 9.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200