TSEN2 Polyclonal Antibody

$Lane 1: Mouse Ovary lysates; Lane 2: Mouse Thymus lysates probed with TSEN2 Polyclonal Antibody, Unconjugated (bs-9735R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Ovary lysates; Lane 2: Mouse Thymus lysates probed with TSEN2 Polyclonal Antibody, Unconjugated (bs-9735R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$25 ug total protein per lane of various lysates (see on figure) probed with TSEN2 polyclonal antibody, unconjugated (bs-9735R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with TSEN2 polyclonal antibody, unconjugated (bs-9735R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Sheep
Horse

Overview
Catalog #	bs-9735R
Product Name	TSEN2 Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Sheep, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TSEN2
Immunogen Range	1-100/465
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	80746
Synonyms	HsSen2; MGC2776; MGC4440; SEN2; SEN2L; tRNA intron endonuclease Sen2; tRNA splicing endonuclease 2 homolog SEN2, S. cerevisiae; tRNA splicing endonuclease subunit Sen2; TSEN 2; SEN2_HUMAN.
Background	The tRNA-splicing endonuclease complex is responsible for identifying and cleaving pre-tRNA at both 5? and 3? splice sites, thereby releasing introns and free tRNA molecules with 2',3' cyclic phosphates and 5'-OH termini. In addition to its role in pre-tRNA splicing, the heterotetrameric endonuclease complex participates in mRNA processing and, via its association with pre-mRNA processing factors, is thought to link pre-tRNA and pre-mRNA splicing events. TSEN2 (tRNA-splicing endonuclease subunit Sen2), also known as tRNA-intron endonuclease Sen2, is a 465 amino acid nuclear protein that constitutes one of the two catalytic subunits of the tRNA-splicing endonuclease complex. There are three isoforms of TSEN2 that are produced as a result of alternative splicing events. Isoform 1 seems to carry the active site for 5?-splice site cleavage. Defects in the gene encoding TSEN2 are the cause of pontocerebellar hypoplasia type 2B, which is characterized by progressive microencephaly with epilepsy, extrapyramidal dyskinesia and chorea without spinal cord findings.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200