AMPK beta 2

$Lane 1: Mouse Brain tissue lysates; Lane 2: Rat Brain tissue lysates probed with AMPK beta 2 Monoclonal Antibody, Unconjugated (bsm-33866M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Brain tissue lysates; Lane 2: Rat Brain tissue lysates probed with AMPK beta 2 Monoclonal Antibody, Unconjugated (bsm-33866M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bsm-33866m
Product Name	AMPK beta 2
Applications	WB IHC-P IHC-F IF
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human AMPK beta 2
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5565
Swiss Prot	O43741
Subcellular location	Cytoplasm, Nucleus
Synonyms	AMP activated protein kinase beta 2 non catalytic subunit; AMPK beta 2; AMPK beta 2 chain; PRKAB 2; Protein kinase AMP activated beta 2 non catalytic subunit; AAKB2_HUMAN; AMPK b2; AMPK-b2.
Background	AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. Also regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone-sensitive lipase. This is a regulatory subunit, may be a positive regulator of AMPK activity. It may also serve as an adapter molecule for the catalytic alpha-subunit.
Application Dilution
WB	=1:1000-2000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500