BRAF Monoclonal Antibody

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human Raji cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human MOLT4 cell lysates; Lane 7: Human HL-60 cell lysates probed with B-Raf Monoclonal Antibody, Unconjugated (bsm-34218M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human Raji cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human MOLT4 cell lysates; Lane 7: Human HL-60 cell lysates probed with B-Raf Monoclonal Antibody, Unconjugated (bsm-34218M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IP
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-34218m
Product Name	BRAF Monoclonal Antibody
Applications	WB ELISA IHC-P IHC-F IP IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human B-Raf protein
Clonality	Monoclonal
Clone #	3G9
Isotype	IgG2a κ
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	673
Swiss Prot	P15056
Synonyms	94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF 1; Braf; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; p94; RAFB 1; RAFB1; v raf murine sarcoma viral oncogene homolog B1; FLJ95109; BRAF_HUMAN.
Application Dilution
WB	=1:100-1000
ELISA	ELISA=1:30-3000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:400-800
IP	IP=1:100-500
IF	IF=1:50-500