MMP14 (3A6) Monoclonal Antibody

$HT-1080 Cells lysates probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.$

HT-1080 Cells lysates probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.

$Lane 1: A2058 Cell lysates; probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A2058 Cell lysates; probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 cells; Antibody incubation with MMP14 (3A6) Monoclonal Antibody, Unconjugated (bsm-51028M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary DAPI and for nuclei staining.$

Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 cells; Antibody incubation with MMP14 (3A6) Monoclonal Antibody, Unconjugated (bsm-51028M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary DAPI and for nuclei staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-51028M
Product Name	MMP14 (3A6) Monoclonal Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MMP14 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 470-499 amino acids from the C-terminal region of human MMP14.
Clonality	Monoclonal
Clone #	3A6
Isotype	IgG
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4323
Swiss Prot	P50281
Synonyms	MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; Matrix metalloproteinase-14; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14
Background	Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2.
Application Dilution
WB	1:300-5000
IHC-P	FCM1:20-100
IHC-F	IF
IF