MASTL (5C3) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51071M |
| Product Name | MASTL (5C3) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This MASTL monoclonal antibody is generated from mouse immunized with MASTL recombinant protein. |
| Clonality | Monoclonal |
| Clone # | 5C3 |
| Isotype | IgG1 |
| Concentration | 0.5ug/ul |
| Purification | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 84930 |
| Swiss Prot | Q96GX5 |
| Synonyms | GW; GWL; THC2; MAST-L; GREATWALL; Serine/threonine-protein kinase greatwall; hGWL; Microtubule-associated serine/threonine-protein kinase-like; MASTL |
| Background | Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. |
| Application Dilution | |
| WB | 1:300-5000 |