EIF4B (5C5) Monoclonal Antibody

$Lane 1: A431; Lane 2: HeLa cell lysate at 20 \u00b5g per lane. probed with bsm-51102M EIF4B (355CT12.6.5) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: A431; Lane 2: HeLa cell lysate at 20 \u00b5g per lane. probed with bsm-51102M EIF4B (355CT12.6.5) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

$Jurkat cell lysate probed with bsm-51102M EIF4B (355CT12.6.5) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Jurkat cell lysate probed with bsm-51102M EIF4B (355CT12.6.5) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51102M
Product Name	EIF4B (5C5) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This EIF4B monoclonal antibody is generated from mouse immunized with EIF4B recombinant protein.
Clonality	Monoclonal
Clone #	5C5
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	1975
Swiss Prot	P23588
Synonyms	EIF-4B; PRO1843; Eukaryotic translation initiation factor 4B; EIF4B
Background	Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Application Dilution
WB	1:300-5000