PODXL (2C4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51201M |
| Product Name | PODXL (2C4) Monoclonal Antibody |
| Applications |
WB
IHC-P
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This PODXL antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL. |
| Clonality | Monoclonal |
| Clone # | 2C4 |
| Isotype | IgG1 |
| Concentration | 0.5ug/ul |
| Purification | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 5420 |
| Swiss Prot | O00592 |
| Synonyms | PC; PCLP; Gp200; PCLP-1; Podocalyxin; GCTM-2 antigen; Podocalyxin-like protein 1; PODXL; PCLP1 |
| Background | Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IF | |