CYP1A1 (10G3) Monoclonal Antibody

$Jurkat lysate (20\u00b5g) probed with bsm-51210M CYP1A1 (10G3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Jurkat lysate (20\u00b5g) probed with bsm-51210M CYP1A1 (10G3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Lane 1: Siha cell lysates; Lane 2: A549 cell lysates probed with CYP1A1 (10G3) Monoclonal Antibody, Unconjugated (bsm-51210M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human

Overview
Catalog #	bsm-51210M
Product Name	CYP1A1 (10G3) Monoclonal Antibody
Applications	WB ELISA
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This CYP1A1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 377-405 amino acids from the C-terminal region of human CYP1A1.
Clonality	Monoclonal
Clone #	10G3
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1543
Swiss Prot	P04798
Synonyms	AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX; Cytochrome P450 1A1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A1
Background	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Application Dilution
WB	1:300-5000
ELISA