CDH1 (3C3) Monoclonal Antibody

$Lane 1: WiDr; Lane 2: mouse stomach lysate at 20 \u00b5g per lane, probed with bsm-51214M CDH1 (813CT11.1.3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: WiDr; Lane 2: mouse stomach lysate at 20 \u00b5g per lane, probed with bsm-51214M CDH1 (813CT11.1.3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

$Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (813CT11.1.3) Monoclonal Antibody (bsm-51214M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (813CT11.1.3) Monoclonal Antibody (bsm-51214M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51214M
Product Name	CDH1 (3C3) Monoclonal Antibody
Applications	WB IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged CDH1 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	3C3
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	999
Swiss Prot	P12830
Synonyms	UVO; CDHE; ECAD; LCAM; Arc-1; CD324; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CDH1
Background	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400