UCH37 (8G6) Monoclonal Antibody

$Lane 1: 293 Cell lysates; Lane 2: HeLa Cell lysates; Lane 3: 293T Cell lysates; probed with UCH37 (8G6) Monoclonal Antibody, unconjugated (bsm-51223M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293 Cell lysates; Lane 2: HeLa Cell lysates; Lane 3: 293T Cell lysates; probed with UCH37 (8G6) Monoclonal Antibody, unconjugated (bsm-51223M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51223M
Product Name	UCH37 (8G6) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged UCH37 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	8G6
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	51377
Swiss Prot	Q9Y5K5
Synonyms	UCH37; CGI-70; INO80R; UCH-L5; Ubiquitin carboxyl-terminal hydrolase isozyme L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5; UCHL5; AD-019
Background	Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
Application Dilution
WB	1:300-5000