MBD3 (5C3) Monoclonal Antibody

$Lane 1: K562 Cell lysates; probed with MBD3 (5C3) Monoclonal Antibody, unconjugated (bsm-51226M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cell lysates; probed with MBD3 (5C3) Monoclonal Antibody, unconjugated (bsm-51226M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51226M
Product Name	MBD3 (5C3) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged MBD3 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	5C3
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	53615
Swiss Prot	O95983
Synonyms	Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3; MBD3
Background	Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
Application Dilution
WB	1:300-5000