Cathepsin D (7B4) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CTSD (892CT11.1.1) Monoclonal Antibody (bsm-51237M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CTSD (892CT11.1.1) Monoclonal Antibody (bsm-51237M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-51237M
Product Name	Cathepsin D (7B4) Monoclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged CTSD protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1509
Swiss Prot	P07339
Synonyms	CPSD; CLN10; HEL-S-130P; Cathepsin D; CTSD
Background	Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Application Dilution
WB	1:300-5000
ELISA	IHC-P1:200-400
IHC-P
IHC-F
IF