CDK4 (9C3) Monoclonal Antibody

$Lane 1: A431 Cell lysates; Lane 2: HeLa Cell lysates; Lane 3: K562 Cell lysates; Lane 4: MCF-7 Cell lysates; Lane 5: NIH\/3T3 Cell lysates; probed with CDK4 (9C3) Monoclonal Antibody, unconjugated (bsm-51314M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A431 Cell lysates; Lane 2: HeLa Cell lysates; Lane 3: K562 Cell lysates; Lane 4: MCF-7 Cell lysates; Lane 5: NIH\/3T3 Cell lysates; probed with CDK4 (9C3) Monoclonal Antibody, unconjugated (bsm-51314M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDK4 (9C3) Monoclonal Antibody (bsm-51314M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining$

Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDK4 (9C3) Monoclonal Antibody (bsm-51314M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining

Applications

WB
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51314M
Product Name	CDK4 (9C3) Monoclonal Antibody
Applications	WB IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This CDK4 antibody is generated from a mouse immunized with a recombinant protein.
Clonality	Monoclonal
Clone #	9C3
Isotype	IgG2a
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	1019
Swiss Prot	P11802
Synonyms	CMM3; PSK-J3; Cyclin-dependent kinase 4; Cell division protein kinase 4; CDK4
Background	Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400