RAB27A (9C6) Monoclonal Antibody

$Lane 1: K562 Cell lysates; probed with RAB27A (9C6) Monoclonal Antibody, unconjugated (bsm-51334M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cell lysates; probed with RAB27A (9C6) Monoclonal Antibody, unconjugated (bsm-51334M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

K562 cells probed with using RAB27A (9C6) Monoclonal Antibody bsm-51334M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:200 (green) compared to an isotype control of mouse IgG1 (blue).

Lane 1: Human K562 cell lysates; Lane 2: Human Jurkat cell lysates probed with RAB27A Monoclonal Antibody, Unconjugated (bsm-51334M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51334M
Product Name	RAB27A (9C6) Monoclonal Antibody
Applications	WB FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A.
Clonality	Monoclonal
Clone #	9C6
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5873
Swiss Prot	P51159
Synonyms	GS2; RAM; RAB27; HsT18676; Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram; RAB27A
Background	Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Application Dilution
WB	1:300-5000
FCM	1:20-100