FZR (1C7) Monoclonal Antibody

$Lane 1: 293T\/17; Lane 2: Hela cell lysates probed with FZR (1C7) Polyclonal Antibody, unconjugated (bsm-51337M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293T\/17; Lane 2: Hela cell lysates probed with FZR (1C7) Polyclonal Antibody, unconjugated (bsm-51337M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51345M
Product Name	FZR (1C7) Monoclonal Antibody
Applications	WB IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This FZR antibody is generated from a mouse immunized with a recombinant protein between1-496 amino acids from human FZR.
Clonality	Monoclonal
Clone #	1C7
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	51343
Swiss Prot	Q9UM11
Synonyms	FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C; Fizzy-related protein homolog; CDC20-like protein 1; Cdh1/Hct1 homolog; FZR1; FYR; KIAA1242
Background	Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Application Dilution
WB	1:300-5000
IF