MYO18B (3C5) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$A431 cells probed with using MYO18B (3C5) Monoclonal Antibody (green) unconjugated bsm-51376M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2b (blue).$

A431 cells probed with using MYO18B (3C5) Monoclonal Antibody (green) unconjugated bsm-51376M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2b (blue).

$Recombinant protein lysates probed with MYO18B (3C5) Polyclonal Antibody, unconjugated (bsm-51376M) at 1:16000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Recombinant protein lysates probed with MYO18B (3C5) Polyclonal Antibody, unconjugated (bsm-51376M) at 1:16000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-51376M
Product Name	MYO18B (3C5) Monoclonal Antibody
Applications	WB FCM IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MYO18B antibody is generated from a mouse immunized with recombinant protein from human MYO18B.
Clonality	Monoclonal
Clone #	3C5
Isotype	IgG2b
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	84700
Swiss Prot	Q8IUG5
Synonyms	KFS4; Unconventional myosin-XVIIIb; MYO18B
Background	May be involved in intracellular trafficking of the muscle cell when in the cytoplasm, whereas entering the nucleus, may be involved in the regulation of muscle specific genes. May play a role in the control of tumor development and progression; restored MYO18B expression in lung cancer cells suppresses anchorage-independent growth.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400