Histone H3 (9C2) Monoclonal Antibody

$Lane 1: C6 Cell Lysates; Lane 2: HeLa Cell Lysates; probed with Histone H3 (809CT10.4.2) Monoclonal Antibody, unconjugated (bsm-51431M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.$

Lane 1: C6 Cell Lysates; Lane 2: HeLa Cell Lysates; probed with Histone H3 (809CT10.4.2) Monoclonal Antibody, unconjugated (bsm-51431M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.

Applications

Reactivity

Human
Rat

Overview
Catalog #	bsm-51431M
Product Name	Histone H3 (9C2) Monoclonal Antibody
Applications	WB
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This Histone H3 antibody is generated from a mouse immunized with Histone H3 recombinant protein.
Clonality	Monoclonal
Clone #	9C2
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	8350
Swiss Prot	P68431
Synonyms	H3/A; H3FA; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB	1:300-5000