ATG7 Monoclonal Antibody

$Lane 1: Recombinant APG7 protein probed with ATG7 Monoclonal Antibody, Unconjugated (bsm-51461M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Recombinant APG7 protein probed with ATG7 Monoclonal Antibody, Unconjugated (bsm-51461M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Recombinant protein

Predicted Reactivity

Human

Overview
Catalog #	bsm-51461m
Product Name	ATG7 Monoclonal Antibody
Applications	WB
Reactivity	Recombinant protein
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human APG7 (His-tagged).
Clonality	Monoclonal
Clone #	AT01
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10533
Swiss Prot	O95352
Subcellular location	Cytoplasm
Synonyms	Apg 7; APG7 autophagy 7 like; APG7 autophagy 7-like (S. cerevisiae); APG7 like; APG7, S. cerevisiae, homolog of; APG7L; ATG 7; atg7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7 autophagy related 7 homolog; Atg7l; Autophagy 7, S. cerevisiae, homolog of; Autophagy related protein 7; Autophagy-related 7 (yeast); DKFZp434N0735; GSA 7; GSA7; hAGP7; Ubiquitin activating enzyme E1 like protein; ATG7_HUMAN.
Background	Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3.
Application Dilution
WB	=1:500-2000