ALDH6A1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: MCF-7 cell lysates; Lane 2: T47D cell lysate probed with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: MCF-7 cell lysates; Lane 2: T47D cell lysate probed with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51469m
Product Name	ALDH6A1 Monoclonal Antibody
Applications	WB IHC-P IHC-F IF ICC/IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human ALDH6A1.
Clonality	Monoclonal
Clone #	AL06
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4329
Swiss Prot	Q02252
Subcellular location	Nucleus
Synonyms	Aldehyde dehydrogenase 6 family member A1; Aldehyde dehydrogenase family 6 member A1; Aldehyde dehydrogenase family 6, subfamily A1; Malonate semialdehyde dehydrogenase; Malonate-semialdehyde dehydrogenase [acylating]; Methylmalonate semialdehyde dehydrogenase [acylating] mitochondrial; Methylmalonate-semialdehyde dehydrogenase [acylating]; MGC37325; MGC40271; Mitochondrial acylating methylmalonate semialdehyde dehydrogenase; mitochondrial; MMSA_HUMAN; MMSADHA; MMSDH;
Background	Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:25
IHC-F	IHC-F=1:400-800
IF	IF=1:25
ICC/IF	ICC/IF=1:25