PSMA5 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: K562 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: K562 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: Human liver tissue lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: MCF-7 cell lysates;Lane 5: Neuro-2a cell lysates; Lane 6: PC-12 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human liver tissue lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: MCF-7 cell lysates;Lane 5: Neuro-2a cell lysates; Lane 6: PC-12 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Predicted Reactivity

Human
Mouse
Rat
Cow

Overview
Catalog #	bsm-51520m
Product Name	PSMA5 Monoclonal Antibody
Applications	WB IHC-P IHC-F IF ICC/IF
Predicted Reactivity	Human, Mouse, Rat, Cow
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human PSMA5 (Fragment) (His-tagged).
Clonality	Monoclonal
Clone #	N9G6
Isotype	IgG1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5686
Swiss Prot	P28066
Subcellular location	Cytoplasm, Nucleus
Synonyms	Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome (prosome macropain) subunit alpha type 5; Proteasome alpha5 subunit; Proteasome component 5; Proteasome subunit alpha type 5; Proteasome subunit alpha type-5; Proteasome subunit zeta; Proteasome zeta chain; PSA5_HUMAN; PSC5; ZETA; Proteasome 20S alpha 5/PSMA5.
Background	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:400-800
IHC-F	IHC-F=1:400-800
IF	IF=1:10-50
ICC/IF	ICC/IF=1:25